Power and Sample Size Calculation for Differential Abundance Microbiome Studies

Provides functions for estimating statistical power and required sample sizes in differential abundance microbiome studies using negative binomial models. The methods are based on Agronah and Bolker (2025) . The package includes tools for simulation-based power analysis and sample size estimation using generalized additive models (GAMs), and visualization utilities for exploring the relationship between power, effect size, abundance, and sample size.

power.nb R package

Introduction

power.nb is an R package with the following features

• power.nb has two simulators for simulating microbiome data

• A framework for statistical power and sample size calculation for differential abundance microbiome studies

Microbiome data simulation